The platelet ATP release kinetics test is used to assess platelet viability and function in clinical and research settings. This test provides valuable information on platelet activation by measuring the release of ATP from dense granules upon stimulation. Since the test is performed in whole blood, it eliminates the need for extensive preparation steps, making it a more efficient and streamlined process. Platelet ATP release kinetics is planned to be used to evaluate platelet health, monitor platelet function in various conditions (such as thrombocytopathy, cardiovascular disease, and diabetes), and assess the effects of medications or treatments on platelet function.
Platelets in platelet-rich plasma or whole blood are activated using a specific stimulus, causing them to release ATP from their dense granules. This activation mimics natural physiological conditions and triggers the release of ATP, which is crucial for assessing platelet function.
The released ATP is quantified using a luciferase/luciferin reaction. Luciferase catalyses the conversion of luciferin in the presence of ATP, producing light. The intensity of the emitted light (luminescence) is directly proportional to the amount of ATP present in the sample. This luminescence is measured, providing a sensitive and accurate assessment of platelet activation and vitality.
Due to the kinetics of the reaction, additional parameters such as the maximum rate of ATP release (Vmax) and the time to reach Vmax (time to Vmax) can also be analysed. The time to Vmax may serve as a substitute for the traditional lag time, offering a more precise measure of platelet activation dynamics. These kinetic parameters provide further insights into the efficiency and responsiveness of platelets, enhancing the overall evaluation of platelet function.
